Since the publication of High-Resolution Electrophorsesis and Immunofixation 2e, there have been ever-increasing advances in the analyses of proteins, by electrophoresis in particular. Protein Electrophoresis in Clinical Diagnosis shows the changes in both techniques and interpretation, presenting a comprehensive review of serum protein techniques, immunofixation techniques, approaches to pattern interpretation, and pattern interpretation in both cerebralspinal fluid and urine. Conditions associated with Monoclunal Gammopathies are considered, as are the appropriate strategies for their detection. David Keren is well-known as the leader in this field, his work on guidelines becoming the benchmark for all those involved in protein detection in serum and urine. Dr Keren's book will be essential in every laboratory, and read by pathologists, chemical chemists, medical technicians and clinicians (particularly hematologists and oncologists).
This book provides a comprehensive and up-to-date review of recent trends of green science and technology. Worldwide deterioration of environment and global warming threaten our lifestyle and the survival of all creatures. In order to weather these problems, we need to construct a multidisciplinary approach involving the fusion of various advanced researches. The book begins with an overview on fundamental research about generation and utilization of renewable energy, protection of the earth's ecosystem for better coexistence with nature, development of artificial intelligence-based agriculture and molecular recognitionbased welfare and covers a wide range of innovative research on green science and technology.
Electrophoresis is a straightforward but informative analytical method used in biochemistry, biology and medicine. This book combines a detailed discussion of theory and technical application with an elaborate section on troubleshooting and problem solving in electrophoresis. Therefore the book is an important guide for both students and scientists.
Two-Dimensional Gel Electrophoresis of Proteins: Methods and Applications reviews current methods and clinical applications of two-dimensional gel electrophoresis of proteins, including the QUEST system, silver staining, and peptide mapping. Two-dimensional gel electrophoresis are applied to the study of diseases ranging from inborn errors of metabolism to human germ-line mutation rates, cancer, and mistranslation in animal and bacterial cells. This volume is organized into three sections encompassing 14 chapters and begins with an overview of the methodology of two-dimensional gel electrophoresis, followed by a discussion of computerized two-dimensional gel electrophoresis, silver staining, immunoblotting, and one- and two-dimensional peptide mapping. In most cases, a step-by-step guide to the techniques is given so that procedures may be easily repeated. A catalog of mouse fibroblast proteins is also given. The chapters that follow focus mainly on applications of two-dimensional gel electrophoresis in areas such as clinical and cancer research, human genetics, protein biosynthesis, and gene expression in plants. The final section presents current protein catalogs of Escherichia coli and human HeLa cells. This book is suitable for young researchers as well as for senior scientists working with a wide variety of problems in molecular and cell biology, basic biochemistry, genetics, and clinical research.
High-Resolution Electrophoresis and Immunofixation: Techniques and Interpretation, Second Edition presents the important developments in the technology of serum protein electrophoresis. This book discusses the electrophoretic patterns that one encounters when using high-resolution electrophoresis and immunofixation. Organized into nine chapters, this edition begins with an overview of the migration of charged particles in an electrical field. This text then presents the study of nasal and aural fluids looking for cerebrospinal fluid (CSF)-specific transferrin to detect CSF leakage due to a skull fracture or tumor. Other chapters consider some detailed studies of strategies employing immunoglobulin quantification and high-resolution electrophoresis to detect monoclonal gammopathies. This book discusses as well the three available strategies to enhance the efficiency of diagnosis of monoclonal gammopathies. The final chapter deals with the principles of high-resolution electrophoresis and immunofixation electrophoresis (IFE). This book is a valuable resource for pathologists, technologists, epidemiologists, physicians, and clinicians.
With the end of the Human Genome Project in sight, the next important step is to determine the function of genes. Proteome Research is an important approach to this study and is the first book to comprehensively cover the application of two-dimensional electrophoresis, the central methodology in proteome research. The state-of-the-art is described in detail and the available detection methods are extensively covered. Sufficient detail is given to allow readers to apply these technologies to their own particular requirements.
All pathology residents must have a good command of clinical chemistry, toxicology, immunology, and laboratory statistics to be successful pathologists, as well as to pass the American Board of Pathology examination. Clinical chemistry, however, is a topic in which many senior medical students and pathology residents face challenges. Clinical Chemistry, Immunology and Laboratory Quality Control meets this challenge head on with a clear and easy-to-read presentation of core topics and detailed case studies that illustrate the application of clinical chemistry knowledge to everyday patient care. This basic primer offers practical examples of how things function in the pathology clinic as well as useful lists, sample questions, and a bullet-point format ideal for quick pre-Board review. While larger textbooks in clinical chemistry provide highly detailed information regarding instrumentation and statistics, this may be too much information for students, residents, and clinicians. This book is designed to educate senior medical students, residents, and fellows, and to "refresh" the knowledge base of practicing clinicians on how tests are performed in their laboratories (i.e., method principles, interferences, and limitations). Takes a practical and easy-to-read approach to understanding clinical chemistry and toxicology Covers all important clinical information found in larger textbooks in a more succinct and easy-to-understand manner Covers essential concepts in instrumentation and statistics in such a way that fellows and clinicians understand the methods without having to become specialists in the field Includes chapters on drug-herb interaction and pharmacogenomics, topics not covered by textbooks in the field of clinical chemistry or laboratory medicine
Electrophoresis methods are simple and informative analytical methods, which are used in biochemistry and medicine. They are necessary for precise investigation of proteins and nucleic acids (DNA and RNA). Electrophoresis is practiced in chemical, biochemical, and clinical laboratories, as well as in forensic medicine laboratories. This book will discuss the fundamentals of electrophoresis methods at the graduate level.
This fifth edition of the successful, long-selling classic has been completely revised and expanded, omitting some topics on obsolete DNA electrophoresis, but now with a completely new section on electrophoretic micro-methods and on-the-chip electrophoresis. The text is geared towards advanced students and professionals and contains extended background sections, protocols and a trouble-shooting section. It is now also backed by a supplementary website providing all the figures for teaching purposes, as well as a selection of animated figures tested in many workshops to explain the underlying principles of the different electrophoretic methods.
This textbook will provide a comprehensive, state-of-the art review the field of diagnostic hematopathology as it’s applied to patients with plasma cell neoplasms. Particular emphasis will be placed on immunophenotypic data – immunohistochemistry and flow cytometry – as well as cytogenetics. We will also discuss how these ancillary data can predict prognosis and chemotherapeutic response. Plasma Cell Neoplasms will serve as a very useful resource for physicians and researchers interested in the plasma cell myeloma diagnosis, therapy, and research. It will provide a concise yet comprehensive summary of the current status of the field that will help guide patient management and stimulate investigative efforts. All chapters will be written by experts in their fields and will include the most up to date scientific and clinical information.